Analysis of protection afforded by a Clostridium perfringens α-toxoid against heterologous clostridial phospholipases C

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• 作者：Brendan N. Neeson ; Graeme C. Clark ; Helen S. Atkins ; Bryan Lingard ; Richard W. Titball
• 关键词：Clostridium perfringens ; Phospholipase C ; Myonecrosis ; Vaccine
• 刊名：Microbial Pathogenesis
• 出版年：2007
• 期刊代码：172_08824010
• 类别：bio
• 出版时间：October 2007
• 卷：43
• 期：4
• 页码：161-165
• 文件大小：138 K

摘要

The major virulence determinant in clostridial myonecrosis caused by Clostridium perfringens is a phospholipase C (PLC), the α-toxin. Previously, mice have been protected against challenge with heterologous α-toxin or Clostridium perfringens spores by immunisation with the C-domain (known as Cpa_247–370 or α-toxoid) of the α-toxin. In this study, we have determined the ability of the α-toxoid to protect against the lethal effects of a divergent C. perfringens α-toxin and against the PLCs of C. absonum or C. bifermentans, species which have been isolated from cases of clostridial myonecrosis. Protection against the C. perfringens α-toxin variant, the C. absonum α-toxin or the C. bifermentans PLC was elicited by immunisation with the α-toxoid in vivo.