Identification of expressed genes in cDNA library of hemocytes from the RLO-challenged oyster, Crassostrea ariakensis Gould with special functional implication of three complement-related fragments (CaC1q1, CaC1q2 and CaC3)

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• 作者：Ting Xu^a ; Jiasong Xie^a ; Jianming Li^b ; Ming Luo^a ; Shigen Ye^a ; Xinzhong Wu^a ; ^{wuxz@zju.edu.cn}
• 关键词：Crassostrea ariakensis Gould ; Rickettsia-like organism (RLO) ; cDNA library ; C1q-domain-containing protein ; Complement component C3-like gene
• 刊名：Fish & Shellfish Immunology
• 出版年：2012
• 期刊代码：115_10504648
• 类别：abs
• 出版时间：June, 2012
• 卷：32
• 期：6
• 页码：1106-1116
• 文件大小：1690 K

摘要

A SMARTer鈩?cDNA library of hemocyte from Rickettsia-like organism (RLO) challenged oyster, Crassostrea ariakensis Gould was constructed. Random clones (400) were selected and single-pass sequenced, resulted in 200 unique sequences containing 96 known genes and 104 unknown genes. The 96 known genes were categorized into 11 groups based on their biological process. Furthermore, we identified and characterized three complement-related fragments (CaC1q1, CaC1q2 and CaC3). Tissue distribution analysis revealed that all of three fragments were ubiquitously expressed in all tissues studied including hemocyte, gills, mantle, digestive glands, gonads and adductor muscle, while the highest level was seen in the hemocyte. Temporal expression profile in the hemocyte monolayers reveled that the mRNA expression levels of three fragments presented huge increase after the RLO incubation at 3聽h and 6聽h in post-challenge, respectively. And the maximal expression levels at 3聽h in post-challenge are about 256, 104 and 64 times higher than the values detected in the control of CaC1q1, CaC1q2 and CaC3, respectively.