Low level of Fc纬RIII expression on murine natural killer cells
- 作者：Markus Biburger ; Falk Nimmerjahn ; fnimmerj@biologie.uni-erlangen.de
- 关键词：Immunoglobulin G ; NK cells ; Fc receptors ; Macrophage ; Monocytes ; ADCC
- 刊名：Immunology Letters
- 出版年：2012
- 期刊代码：115_01652478
- 类别：med
- 出版时间：30 March, 2012
- 卷：143
- 期：1
- 页码：53-59
- 文件大小：1666 K
摘要
Cellular Fc纬-receptors are crucial for mediating the functions of therapeutic antibodies. Antibody dependent cellular cytotoxicity (ADCC) is an important mechanism by which Fc纬-receptor expressing cells of the innate immune system including natural killer (NK) cells can kill opsonized target cells. During FACS analysis, however, binding of the Fc-fragment of staining antibodies specific for cell type specific receptors can lead to false positive results and wrong interpretation of the data. Current strategies to block such unwanted binding largely target Fc纬RIIB and Fc纬RIII but not the recently identified mouse Fc纬RIV. In this study we demonstrate that Fc-dependent binding of the NK cell specific antibody NK1.1 by Fc纬RIV on monocytes results in a large overestimation of Fc纬RIII expression on murine NK cells. These results highlight the importance of blocking unwanted binding of FACS antibodies to Fc纬RIV and shed new light on the expression level of Fc纬RIII on NK cells in mice during the steady state.