Two novel N-terminal coding exons of Prkar1b gene of mouse: Identified using a novel approach of in silico and molecular biology techniques

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• 作者：Abdul Rouf Banday ; Shafquat Azim ; Sayeed Ur Rehman ; Mohammad Tabish ; ^{tabish.bcmlab@gmail.com}
• 关键词：PKA ; Protein kinase A ; AKAPs ; A Kinase Anchoring Proteins ; C ; Catalytic subunit ; R ; Regulatory subunit ; RT-PCR ; Reverse transcriptase polymerase chain reaction ; EST ; Expressed sequence tags ; PN ; Postnatal ; R1尾 ; Regulatory type 1 beta subunit
• 刊名：Gene
• 出版年：2012
• 期刊代码：73_03781119
• 类别：bio
• 出版时间：25 May, 2012
• 卷：500
• 期：1
• 页码：73-79
• 文件大小：1336 K

摘要

The Prkar1b gene encodes regulatory type 1 beta subunit of protein kinase A. Here we report that mouse R1尾 gene produces three alternative splice variants (designated as mR1尾1, mR1尾2 and mR1尾3) that have different N-terminal protein structures. New splice variants were identified using combinatorial approach of bioinformatics pipeline involving online available tools and databases, and molecular biology techniques involving RT-PCR, semi-nested PCR and sequencing. Except mR1尾3, which was not detected by RT-PCR in brain and muscle tissues of 3 day old mice, all three spliced isoforms were found to be ubiquitously expressed in tissues and postnatal developmental stages examined. The presence of different N-termini in isoforms may be important for unique docking interactions with A kinase anchoring proteins.