Importance of trimer–trimer interactions for the native state of the plant light-harvesting complex II
- 作者：Petar H. Lambrev ; Zsuzsanna Vá ; rkonyi ; Sashka Krumova ; Lá ; szló ; Ková ; cs ; Yuliya Miloslavina ; Alfred R. Holzwarth ; Győ ; ző ; Garab
- 关键词：Thylakoid membrane ; Light-harvesting complex ; Circular dichroism ; Linear dichroism ; Time-resolved fluorescence
- 刊名：Biochimica et Biophysica Acta (BBA)/Bioenergetics
- 出版年：2007
- 期刊代码：13_00052728
- 类别：bio
- 出版时间：June 2007
- 卷：1767
- 期：6
- 页码：847-853
- 文件大小：318 K
摘要
Aggregates and solubilized trimers of LHCII were characterized by circular dichroism (CD), linear dichroism and time-resolved fluorescence spectroscopy and compared with thylakoid membranes in order to evaluate the native state of LHCII in vivo. It was found that the CD spectra of lamellar aggregates closely resemble those of unstacked thylakoid membranes whereas the spectra of trimers solubilized in n-dodecyl-β,d-maltoside, n-octyl-β,d-glucopyranoside, or Triton X-100 were drastically different in the Soret region. Thylakoid membranes or LHCII aggregates solubilized with detergent exhibited CD spectra similar to the isolated trimers. Solubilization of LHCII was accompanied by profound changes in the linear dichroism and increase in fluorescence lifetime. These data support the notion that lamellar aggregates of LHCII retain the native organization of LHCII in the thylakoid membranes. The results indicate that the supramolecular organization of LHCII, most likely due to specific trimer–trimer contacts, has significant impact on the pigment interactions in the complexes.