Electrochemical potential of Microgramma vaccinifolia rhizome lectin

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• 作者：Giselly Maria de Sá ; Santana^a ; Lidiane Pereira de Albuquerque^a ; Thiago Henrique Napoleã ; o^a ; Sandra Rodrigues de Souza^b ; Luana Cassandra Breitenbach Barroso Coelho^a ; Patrí ; cia Maria Guedes Paiva^a ; ^{ppaivaufpe@yahoo.com.br}
• 关键词：Microgramma vaccinifolia ; Lectin ; Eletrochemical potential ; Ions ; Erythrocytes
• 刊名：Bioelectrochemistry
• 出版年：2012
• 期刊代码：7_15675394
• 类别：ch
• 出版时间：June, 2012
• 卷：85
• 期：Complete
• 页码：56-60
• 文件大小：328 K

摘要

This work reports the isolation of Microgramma vaccinifolia rhizome lectin (MvRL) and the determination of electrochemical potentials of MvRL in the presence of Ca^2 +, Mg^2 + and human type O erythrocytes. MvRL showed the highest specific hemagglutinating activity with human type O erythrocytes and showed a single polypeptide band of 17 kDa on SDS-PAGE. MvRL hemagglutinating activity was neutralized after dialysis with EDTA, and addition of Ca^2 + and Mg^2 + restored the activity. Electrochemical potentials of MvRL in the presence of 100 mM Ca^2 + (882 mV) and 60 mM Mg^2 + (1051 mV) were higher (p < 0.05) than in the presence of only 0.15 M NaCl (247 mV), indicating that the electrochemical system was sensitive to structural and physico-chemical changes promoted by these ions. MvRL potential did not change in the presence of type O erythrocytes. The electrochemical system was able to detect changes in electrochemical potentials of MvRL promoted by Ca^2 + and Mg^2 +, even in a complex environment (human serum supplemented with 40 and 60 mM of these ions). The study reveals that the stimulatory effect of Ca^2 + and Mg^2 + on hemagglutinating activity may be linked to conformational change and/or alterations in surface charge distribution of MvRL.