Histone acetyltransferase HBO1 inhibits NF-κB activity by coactivator sequestration
- 作者：Romuald Contzler ; Alexander Regamey ; Bertrand Favre ; Thierry Roger ; Daniel Hohl and Marcel Huber
- 关键词：Histone acetyltransferase ; Gene regulation ; Coactivators
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2006
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：10 November 2006
- 卷：350
- 期：1
- 页码：208-213
- 文件大小：182 K
摘要
The MYST acetyltransferase HBO1 is implicated in the regulation of DNA replication and activities of transcription factors such as the androgen receptor. Since the androgen receptor and NF-κB transcription factors crossmodulate their transcriptional activity, we investigated whether HBO1 regulates NF-κB signaling. Here, we report that in 293T cells HBO1 reduced dose-dependently NF-κB activity stimulated by TNFα, or by overexpressing p65/RelA, RelB, or cRel. Mutational analysis showed that the N-terminal serine-rich region of HBO1 but not the acetyltransferase function was required for inhibition. Electrophoretic mobility-shift assays demonstrated that HBO1 was neither perturbing the formation of p65/RelA DNA complexes nor binding itself to the κB consensus sequence or to p65/RelA, suggesting that HBO1 reduced NF-κB activity by squelching a cofactor. These data establish a novel function for HBO1 showing that it reduced NF-κB activity by sequestrating an essential coactivator from the NF-κB transcriptional complex.