Effects of dietary supplementation of potential probiotic Pseudomonas aeruginosa VSG-2 on the innate immunity and disease resistance of tropical freshwater fish, Labeo rohita

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• 作者：Sib Sankar Giri^a ; ^{sibsankar_2008@rediffmail.com} ; ^{giribiotek@gmail.com} ; Shib Sankar Sen^b ; V. Sukumaran^a
• 关键词：Dietary ; Pseudomonas aeruginosa VSG-2 ; Labeo rohita ; Innate immunity ; Aeromonas hydrophila
• 刊名：Fish & Shellfish Immunology
• 出版年：2012
• 期刊代码：115_10504648
• 类别：abs
• 出版时间：June, 2012
• 卷：32
• 期：6
• 页码：1135-1140
• 文件大小：397 K

摘要

The effects of dietary Pseudomonas aeruginosa VSG-2 supplementation on innate immunity and protection against Aeromonas hydrophila infection were evaluated in Labeo rohita. Fish were fed for 60 days with control diet or 3 experimental diets containing P.聽aeruginosa VSG-2 at 10⁵, 10⁷, and 10⁹聽cfu聽g^鈭抣, respectively. Various innate immune parameters were examined at 30 and 60 days post-feeding. Fish were challenged with A.聽hydrophila 60 days post-feeding and mortalities were recorded over 10 days post-infection. Dietary supplementation of P.聽aeruginosa VSG-2 significantly increased serum lysozyme and alternative complement pathway (ACP) activities, phagocytosis, and respiratory burst activity in head kidney macrophages of L.聽rohita throughout the experimental period. Superoxide dismutase (SOD) activity significantly increased after 60 days in the groups fed diets containing 10⁷ and 10⁹聽cfu聽g^鈭?聽P aeruginosa. Serum IgM levels were significantly higher in the treatment groups than in the control group after 30 days of feeding; however, the opposite result was observed at 60 days. Moreover, fish fed diets containing 10⁷ and 10⁹聽cfu聽g^鈭? P.聽aeruginosa had significantly higher post-challenge survival rates against A.聽hydrophila infection. Further, P.聽aeruginosa VSG-2 was found to be safe for mammals. These results indicate that dietary P.聽aeruginosa VSG-2 supplementation at 10⁷聽cfu聽g^鈭? can effectively improve innate immunity and disease resistance in L.聽rohita.