Development of microsatellite markers for genetic diversity analysis of Dendrobium loddigesii Rolfe, an endangered orchid in China
- 作者：Xiaoyan Cai
- 关键词：Dendrobium loddigesii ; Orchidaceae ; Microsatellite ; Genetic diversity
- 刊名：Biochemical Systematics and Ecology
- 出版年：2012
- 期刊代码：21_03051978
- 类别：abs
- 出版时间：August, 2012
- 卷：43
- 期：Complete
- 页码：42-47
- 文件大小：168 K
摘要
Dendrobium loddigesii Rolfe is an endangered epiphytic orchid with high medicinal and horticulture value. To estimate genetic diversity and population structure of D. loddigesii, 12 new microsatellite markers of D. loddigesii were developed from an enriched library. A total of 98 alleles were detected with an average of 8.2 alleles per locus. The expected heterozygosity of each SSR locus varied from 0.454 to 0.857 with an average of 0.690. Polymorphism information content (PIC) of each SSR locus ranged from 0.358 to 0.838 with an average of 0.637. A high level of genetic diversity was detected. Analysis of molecular variance showed that the main variation component existed within populations (82.02%) rather than among populations (17.98%). Two main clusters were shown in UPGMA dendrogram using TFPGA, which was consistent with the result of principal coordinate analysis (PCA) using NTSYS. These microsatellite makers will contribute to research on individual identification, genetic diversity, population structure, genome mapping and conservation biology of D. loddigesii.