Production of IL-10, TNF and IL-12 by Peripheral Blood Mononuclear Cells in Mexican Workers Exposed to a Mixture of Benzene-Toluene-Xylene

详细信息	查看全文 | 推荐本文 |

• 作者：Luis Cuauhté ; moc Haro-Garcí ; a^a ; Cuauhté ; moc Arturo Juá ; rez-Pé ; rez^b ; ^{carturojp@gmail.com} ; Guadalupe Aguilar-Madrid^b ; Nadia Mayola Vé ; lez-Zamora^b ; Sergio Muñ ; oz-Navarro^c ; Rommel Chac&oacute ; n-Salinas^d ; Cé ; sar Raú ; l Gonzá ; lez-Bonilla^e ; Claudia Rosa Iturbe-Haro^d ; Iris Estrada-Garcí ; a^d ; Ví ; ctor Hugo Borja-Aburto^f
• 关键词：Benzene&ndash ; Toluene&ndash ; Xylene ; Occupational exposure ; Immune response ; Cytokines
• 刊名：Archives of Medical Research
• 出版年：2012
• 期刊代码：24_01884409
• 类别：med
• 出版时间：January, 2012
• 卷：43
• 期：1
• 页码：51-57
• 文件大小：191 K

摘要

| Figures/TablesFigures/Tables | ReferencesReferences

Background and Aims

Occupational exposure to low-level benzene and the joint action of toluene-xylene probably cause effects on circulating monocytes immune response. We undertook this study to determine relationship between occupational exposure to benzene-toluene-xylene mixture (BTX) and IL-10, TNF and IL-12 production by peripheral blood mononuclear cells.

Methods

Exposure was estimated in 54 workers from a paint company in Mexico City through BTX accumulated potential dose (BTX-APD). Two exposure groups were formed: high and low BTX-APD established with a cutoff point at 鈮?.0 of BTX-APD, as a function of the geometric mean of the estimator鈥檚 value distribution and the higher agreement between BTX-APD 鈮?.0 and the areas referred as using (or not) organic solvents in the work process. IL-10, TNF and IL-12 concentrations were measured with ELISA. Through multiple linear regression models, the production of each of the proposed cytokines and of the whole set was assessed.

Results

Workers with high BTX-APD showed a significant reduction in TNF production (尾聽= 鈭?,196.0 pg/mL; p聽= 0.01); a reduction for IL-10 (尾聽= 鈭?20.3; p聽= 0.13) and IL-12 (尾聽= 鈭?43.3; p聽= 0.09) was also observed, although without statistical significance.

Conclusions

TNF production assessed in workers with a high BTX-APD is lower than in聽those with a low BTX-APD, but not in IL-10 and IL-12 production.