摘要
The internal transcribed spacers (ITS) of the nuclear ribosomal DNA and the chloroplast gene matK were used to characterize 39 accessions of wild roses from nine botanical sections in China. Aims of the study were to define the phylogenetic profiles of species and to provide useful information for the classification. Independent and combined phylogenetic analyses for the ITS and matK sequences were performed using maximum parsimony (MP) method, indicating that: (1) six accessions from the section Pimpinellifoliae formed a clade, and Rosa xanthina was separated from others, which reflected these characters of the species in section Pimpinellifoliae on the molecular level; (2) the morphological similarity between sections Synstylae and Chinenses, was confirmed on the molecular level by our analysis. (3) Rosa praelucens from the section Microphyllae formed a clade with all the accessions of the section Cinnamomeae. This finding suggested that further study of the systematic position R. praelucens based on more DNA data. The results showed that a combining phylogenetic analysis was more helpful to clarify origin and classification of species whose systematic position is still uncertain. Moreover, our results will provide helpful evidences for the classification and evolution of genus Rosa.