Role of toll-like receptors in primary Sj枚gren鈥檚 syndrome with a special emphasis on B-cell maturation within exocrine tissues

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• 作者：Thomas Guerrier^a ; Laë ; titia Le Pottier^a ; Valé ; rie Devauchelle^a ; ^b ; Jacques-Olivier Pers^a ; ^b ; Christophe Jamin^a ; ^b ; ^{christophe.jamin@univ-brest.fr} ; Pierre Youinou^a ; ^b
• 关键词：Sjö ; gren&rsquo ; s syndrome ; B lymphocyte ; Toll-like receptor
• 刊名：Journal of Autoimmunity
• 出版年：2012
• 期刊代码：101_08968411
• 类别：imm
• 出版时间：August, 2012
• 卷：39
• 期：1-2
• 页码：69-76
• 文件大小：2312 K

摘要

Be they follicular cells within the germinal centers (GCs) or marginal zone (MZ), all na茂ve mature B lymphocytes need tonic signaling to stay alive. We reasoned that the same holds true for those B lymphocytes that proliferate in the salivary glands (SGs) of patients with primary Sj枚gren鈥檚 syndrome. Based on B cell infiltration, 11 SGs and three tonsil samples were selected for further examination. Tissue sections were stained using CD20 combined with CD10, CD21, CD27, CD38 or IgD. They were also laser-microdissected for quantitative RT-PCR of transcription factors, GC-specific activation-induced cytidine deaminase (AID) and TLR9. Some B cell aggregates proved to be real GCs according to their membrane markers, whereas others were clusters of transitional type II B cells. These contained mRNAs for Notch-2 and Blimp-1, but not for Pax-5, Bcl-6 and AID. Unanticipated was the finding of mRNAs for TLR9 in these clusters of MZ B-cells, but not in the real GCs. Not only do TLR9 deliver sufficiency of tonic signaling to keep B cells alive, but they also confer autoreactive B cells with an MZ-like phenotype. Thus, TLRs might be targets for forthcoming biotherapies.