Rutin inhibits 尾-amyloid aggregation and cytotoxicity, attenuates oxidative stress, and decreases the production of nitric oxide and proinflammatory cytokines

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• 作者：Shao-wei Wang^a ; ^b ; ¹ ; Yu-Jiong Wang^b ; ¹ ; Ya-jing Su^a ; Wei-wei Zhou^a ; ^c ; Shi-gao Yang^a ; Ran Zhang^a ; Min Zhao^a ; Ya-nan Li^a ; Zi-ping Zhang^b ; Da-wei Zhan^c ; ^{davidzhan@tom.com} ; Rui-tian Liu^a ; ^{rtliu@tsinghua.edu.cn}
• 关键词：AD ; Alzheimer's disease ; A尾 ; 尾-amyloid peptide ; ThT ; thioflavin T ; TEM ; transmission electron microscope ; DCFH-DA ; 2&prime ; 7&prime ; -dichlorfluorescein-diacetate ; MTT ; 3-[4 ; 5-dimethylthiazol-2-yl]-2 ; 5-diphenyltetrazolium bromide ; ROS ; reactive oxygen sp
• 刊名：Neurotoxicology
• 出版年：2012
• 期刊代码：35_0161813x
• 类别：pha
• 出版时间：June, 2012
• 卷：33
• 期：3
• 页码：482-490
• 文件大小：1123 K

摘要

Alzheimer's disease (AD) is a complex, multi-factorial neurodegenerative disease. The aggregation of soluble 尾-amyloid (A尾) into fibrillar deposits is a pathological hallmark of AD. The A尾 aggregate-induced neurotoxicity, inflammatory reactions, oxidative stress, and nitric oxide (NO) generation are strongly linked to the etiology of AD. Here, we show that the common dietary flavonoid, rutin, can dose-dependently inhibit A尾42 fibrillization and attenuate A尾42-induced cytotoxicity in SH-SY5Y neuroblastoma cells. Moreover, rutin decreases the formation of reactive oxygen species (ROS), NO, glutathione disulfide (GSSG), and malondialdehyde (MDA), reduces inducible nitric oxide synthase (iNOS) activity, attenuates mitochondrial damage, increases the glutathione (GSH)/GSSG ratio, enhances the activities of super oxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), and modulates the production of proinflammatory cytokines by decreasing TNF-伪 and IL-1尾 generation in microglia. Taken together, the actions of rutin on multiple pathogenic factors deserves further investigation for the prevention and treatment of AD.