Biosensing invertase-based Langmuir-Schaefer films: Preparation and characteristic
- 作者：Joanna Cabaj ; [Author Vitae] ; Jadwiga So艂oducho ; jadwiga.soloducho@pwr.wroc.pl ; [Author Vitae] ; Agnieszka J臋drychowska ; [Author Vitae] ; Dorota Zaj膮c ; [Author Vitae]
- 关键词：Langmuir&ndash ; Schaefer films ; Invertase ; Phospholipids ; Conducting thianthrenes ; Biosensing effect ; AFM microphotography
- 刊名：Sensors and Actuators B ; Chemical
- 出版年：2012
- 期刊代码：161_09254005
- 类别：et
- 出版时间：20 May, 2012
- 卷：166-167
- 期：Complete
- 页码：75-82
- 文件大小：726 K
摘要
The formation of hybrid films of the industrially important enzyme invertase and fatty lipids under enzyme-friendly conditions is described. The approach involves a simple physical adsorption protocol wherein invertase adsorbs into the preformed cationic phospholipid-octadecylamine Langmuir-Schaefer (LS) film. Immobilization of invertase onto the film is highly pH-dependent, underlining the role of attractive electrostatic interactions between the enzyme and the lipid layer. The stability of the enzyme in the lipid matrix was confirmed by biocatalytic activity measurements. Withal, the sensor sensitization was achieved by an amphiphilic conducting structure - N-hexadecyl-2,8-bis(thianthrene)phenothiazine admixed into the film. The interlaced compound was expected to facilitate the electron transfer as well as to enhance the sensor sensitivity. The control of molecular architecture and choice of a suitable phospholipid-octadecylamine matrix allows invertase-containing Langmuir-Schaefer films to be used in sucrose sensing devices.