The non-random distribution of UV-induced photoproducts in the nuclear matrix and non-matrix DNA fractions

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• 作者：Widlak ; Piotr ; Bykov ; Vladimir J. ; Hemminki ; Kari ; Rzeszowska-Wolny ; Joanna
• 关键词：Ultraviolet radiation ; DNA photoproducts ; Nuclear matrix ; MAR (matrix attached region) sequence
• 刊名：Cancer Letters
• 出版年：1996
• 期刊代码：44_03043835
• 类别：med
• 出版时间：November 29, 1996
• 卷：108
• 期：2
• 页码：215-223
• 文件大小：753 K

摘要

The formation of UV-induced photoproducts in the chromatin fractions of human lymphocytes was studied by ³²P-post-labeling. A higher level of DNA lesions was found in the matrix-attached DNA fraction as compared to non-matrix DNA of irradiated cells (about 150 and 110 adducts per 10⁶ nucleotides, respectively, at a 500 J/m² 254 nm-UV dose). Formation of photoproducts in a MAR (matrix attached region) sequence from the mouse kappa immunoglobulin gene irradiated in vitro was examined as well. The MAR sequence showed a two-fold higher level of adducts as compared to non-MAR DNA. The effect of photoproducts on complex-formation between MAR DNA and proteins of the nuclear matrix was studied in vitro. The amount of UV-induced adducts was 1.5-fold higher in matrix-bound fraction as compared to non-fractionated DNA (and five-fold higher as compared to unbound fraction), which possibly resulted from preferential binding of lesion-containing DNA fragments to the nuclear matrix proteins.