A new hydrogen peroxide biosensor based on synergy of Au@Au<sub>2sub>S<sub>2sub>O<sub>3sub> core-shell nanomaterials and multi-walled carbon nanotubes towards hemoglobin
- 作者：Yuanjun Li<sup>asup> ; Fuying Huang<sup>asup> ; Zhuanxi Luo<sup>bsup> ; Bin Xu<sup>bsup> ; Xinxing Wang<sup>asup> ; Feiming Li<sup>asup> ; Fei Wang<sup>asup> ; Lixia Huang<sup>asup> ; Shunxing Li<sup>asup> ; Yancai Li<sup>asup><sup> ; sup><sup>liyancai2000@yahoo.com.cnsup>
- 关键词：H2O2 biosensor ; Au@Au2S2O3 ; Core@shell nanomaterials ; Hemoglobin ; Multi-walled carbon nanotubes
- 刊名：Electrochimica Acta
- 出版年：2012
- 期刊代码：30_00134686
- 类别：ch
- 出版时间：15 July, 2012
- 卷：74
- 期：Complete
- 页码：280-286
- 文件大小：930 K
摘要
A new H<sub>2sub>O<sub>2sub> biosensor was constructed by immobilizing Hb in the Chit-MWNTs/Au@Au<sub>2sub>S<sub>2sub>O<sub>3sub> composite membrane. Owing to the superior performance of novel Au@Au<sub>2sub>S<sub>2sub>O<sub>3sub> core-shell nanomaterials and its synergy with multi-walled carbon nanotubes (MWNTs), the direct electron transfer of Hb in composite membrane was easily achieved. The Chit-MWNTs/Hb/Au@Au<sub>2sub>S<sub>2sub>O<sub>3sub>/GCE displayed a pair of well-defined and reversible redox peaks in 0.1 M pH 7.0 PBS. The electrocatalytic reduction to H<sub>2sub>O<sub>2sub> of Chit-MWNTs/Hb/Au@Au<sub>2sub>S<sub>2sub>O<sub>3sub>/GCE was also studied. Its apparent Michaelis-Menten constant for H<sub>2sub>O<sub>2sub> was 0.014 mM, showing a good affinity. The experimental results showed that the linear dependence of the biosensor was from 4.0 脳 10<sup>鈭?sup> to 7.2 脳 10<sup>鈭?sup> M, and the detection limit of the sensor was 3.2 脳 10<sup>鈭?sup> M (S/N = 3). Moreover, the biosensor showed a rapid response to H<sub>2sub>O<sub>2sub>, a good stability and reproducibility.