摘要
Currently, the method most used for measuring the maximum specific growth rate (m>渭m><sub>Hmaxsub>) of heterotrophic biomass is by respirometry, using growth batch tests performed at high food/microorganism ratio. No other technique has been suggested, although the former approach was criticized for providing kinetic constants that could be unrepresentative of the original biomass. An alternative method (seed-increments) is proposed, which relies on measuring the initial rates of respiration (m>r<sub>O2sub>m><sub>_inisub>) at different seeding levels, in a single batch, and in the presence of excess readily biodegradable substrate (S<sub>Ssub>). The ASM1-based underlying equations were developed, which showed that m>渭m><sub>Hmaxsub> could be estimated through the slope of the linear function of m>r<sub>O2sub>m><sub>_inisub>路(m>Vm><sub>WWsub> + m>vm><sub>MLsub>) vs m>vm><sub>MLsub> (volume of mixed liquor inoculum); m>Vm><sub>WWsub> represent the wastewater volume added. The procedure was tested, being easy to apply; the postulated linearity was constantly observed and the method is claimed to measure the characteristics of the biomass of interest.