Saccharification of woody biomass using glycoside hydrolases from Stereum hirsutum

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• 作者：Marimuthu Jeya^a ; Dayanand Kalyani^a ; Saurabh Sudha Dhiman^a ; Hoon Kim^b ; Seongmin Woo^c ; Dongwook Kim^a ; ^c ; ^{dongwook@phygen.com} ; Jung-Kul Lee^a ; ^{jkrhee@konkuk.ac.kr}
• 关键词：Glycoside hydrolase ; Pine biomass ; RSM optimization ; Saccharification ; Stereum hirsutum
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：August, 2012
• 卷：117
• 期：Complete
• 页码：310-316
• 文件大小：581 K

摘要

Enzymatic saccharification of woody biomasses was performed using glycoside hydrolases from Stereum hirsutum, a newly isolated fungal strain found to secrete efficient glycoside hydrolases. The strain showed the highest 尾-glucosidase, cellobiohydrolase, endoglucanase, endoxylanase, laccase, and filter paper activity of 10.3, 1.7, 10.3, 29.9, 0.12, and 0.58 U/ml, respectively. Among the various biomasses tested for saccharification, pine biomass produced maximum reducing sugar. Response surface methodology was used to optimize the hydrolysis of pine biomass to achieve the highest level of sugars. The parameters including enzyme, substrate concentration, temperature and pH were found to be critical for the conversion of pine biomass into sugars. Maximum saccharification of 49.7%(435 mg/g-substrate) was obtained after 96 h of hydrolysis. A close agreement between the experimental results and the model predictions was achieved. S. hirsutum could be a good choice for the production of reducing sugars from cellulosic biomasses.