Fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enhancing enzymatic saccharification and hydrogen production

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• 作者：Lei Zhao^a ; Guang-Li Cao^a ; ^b ; ^{glcao1980@yahoo.com.cn} ; Ai-Jie Wang^a ; Hong-Yu Ren^a ; De Dong^b ; Zi-Nan Liu^b ; Xiao-Yu Guan^b ; Cheng-Jiao Xu^a ; Nan-Qi Ren^a ; ^{rnq@hit.edu.cn}
• 关键词：Fungal pretreatment ; Phanerochaete chrysosporium ; Saccharification ; Hydrogen production
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：June, 2012
• 卷：114
• 期：Complete
• 页码：365-369
• 文件大小：301 K

摘要

The feasibility of fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enzymatic saccharification and H₂ production was investigated in this study. Firstly, cornstalk was pretreated with P. chrysosporium at 29 掳C under static condition for 15 d, lignin reduction was up to 34.3%with holocellulose loss less than 10%. Microscopic structure observation combined FTIR analysis further demonstrated that the lignin and crystallinity were decreased. Subsequently, the fungal-pretreated cornstalk was subjected to enzymatic hydrolysis by the crude cellulase from Trichoderma viride to produce fermentable sugars which were then fermented to bio-H₂ using Thermoanaerobacterium thermosaccharolyticum W16. The maximum enzymatic saccharification was found to be 47.3%which was 20.3%higher than the control without pretreatment. Upon fermentation of enzymatic hydrolysate, the yield of H₂ was calculated to be 80.3 ml/g-pretreated cornstalk. The present results suggested the potential of using hydrogen-producing bacteria for high-yield conversion of cornstalk into bio-H₂ integrate with biological pretreatment and enzymatic saccharification.