Cinnamtannin B-1 from bay wood reduces abnormal intracellular Ca2+ homeostasis and platelet hyperaggregability in type 2 diabetes mellitus patients
- 作者：A. Bouaziz ; S. Salido ; P.J. Linares-Palomino ; A. Sanchez ; J. Altarejos ; A. Bartegi ; Giné ; s M. Salido ; Juan A. Rosado
- 关键词：Type 2 diabetes mellitus ; Cinnamtannin B-1 ; Store-operated calcium entry ; Platelets ; Antioxidants ; Aggregation
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2007
- 期刊代码：116_00039861
- 类别：ch
- 出版时间：15 January 2007
- 卷：457
- 期：2
- 页码：235-242
- 文件大小：487 K
摘要
Type 2 diabetes mellitus induces a number of cardiovascular disorders, including platelet hyperactivity and hyperaggregability, which is associated to an increased oxidant production and abnormal cytosolic Ca2+ mobilization. In the present study, we have investigated the effect of cinnamtannin B-1 obtained from bay wood on oxidants production, Ca2+ mobilization and aggregation in platelets from type 2 diabetic donors. Pretreatment of platelets with cinnamtannin B-1 reversed the enhanced oxidants production and Ca2+ mobilization, including Ca2+ entry, evoked by thapsigargin plus ionomycin or thrombin, observed in platelets from diabetic subjects, so that in the presence of cinnamtannin B-1 Ca2+ entry was similar in platelets from healthy and diabetic subjects. In addition, cinnamtannin B-1 reduced thrombin-induced aggregation in platelets from type 2 diabetic subjects. We conclude that cinnamtannin B-1 exerts an effective antioxidant action in platelets from patients with type 2 diabetes mellitus and reverses the enhanced Ca2+ mobilization and hyperaggregability.