Liuwei Dihuang decoction facilitates the induction of long-term potentiation (LTP) in senescence accelerated mouse/prone 8 (SAMP8) hippocampal slices by inhibiting voltage-dependent calcium channels (VDCCs) and promoting N-methyl-d-aspartate receptor (NMD

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• 作者：Yan Huang¹ ; Heping Zhang¹ ; Sheng Yang ; Haifa Qiao ; Wenxia Zhou ; ^{zhouwx@nic.bmi.ac.cn} ; Yongxiang Zhang ; ^{zhangyx@nic.bmi.ac.cn}
• 关键词：N-methyl-d-aspartate receptor (NMDA) receptor ; Voltage-dependent calcium channel (VDCC) ; Long-term potentiation (LTP) ; Liuwei Dihuang Decotion (LW) ; Senescence accelerated mouse/prone 8 (SAMP8)
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：27 March, 2012
• 卷：140
• 期：2
• 页码：384-390
• 文件大小：689 K

摘要

<h4 class="h4">Ethnopharmacological relevanceh4>Liuwei Dihuang decoction (LW) is a typical traditional Chinese medicine (TCM) prescription and consists of six herbs including Rehmannia glutinosa Libosch. (family: Scrophulariaceae), Cornus officinalis Sieb. (family: Cornaceae), Dioscorea opposite Thunb. (family: Dioscoreaceae), Alisma orientale (G. Samuelsson) Juz (family: Alismataceae), Poria cocos (Schw.)Wolf (family: Polyporaceae) and Paeonia suffruticosa Andrews (family: Paeoniaceae). It has long been used clinically in treatment of many kinds of diseases with the sign of Yin insufficiency of kidney.<h4 class="h4">Aim of the studyh4>

Our previous pharmacological studies demonstrated that LW possesses effect of ameliorating the decline of the learning and memory in senescence accelerated mouse/prone 8 (SAMP8), but the mechanism has not been well established. LW-containing serum (LWCS) is used in the current study to elucidate the possible mechanisms.<h4 class="h4">Materials and methodsh4>

6-month-old SAMP8 was used in this study to investigate the effect of LWCS on the induction of long-term potentiation (LTP). Primary cultured hippocampal neurons were used in this study to investigate the effects of LWCS on [Ca²⁺]_i, I_Ca and N-Methyl-d-aspartate (NMDA)-evoked currents. [Ca²⁺]_i was imaged using Fluo-3 and whole-cell patch recordings were applied to study the I_Ca and NMDA-evoked currents.<h4 class="h4">Resultsh4>

We find that LWCS facilitates the induction of LTP in hippocampal slices of 6-month-old SAMP8. In primary cultured hippocampal neurons, LWCS increases intracellular [Ca²⁺]_i, the I_Ca is suppressed by LWCS, and NMDA-evoked currents are promoted.<h4 class="h4">Conclusionh4>

These results indicate that LW improves the synaptic plasticity by inhibiting voltage-dependent calciumchannels (VDCCs) and promoting the function of NMDA receptors. This improvement might be one of the mechanisms contributing to cognitive improvement effect of LW.