In vitro and in vivo studies on the antioxidant activity of fish peptide isolated from the croaker (Otolithes ruber) muscle protein hydrolysate
- 作者：R.A. Nazeer ; nazeer.ra@ktr.srmuniv.ac.in ; ra_nazeer@yahoo.com ; N.S. Sampath Kumar ; R. Jai Ganesh
- 关键词：Otolithes ruber ; Antioxidant ; Lipid peroxidation ; DNA damage ; Peptide
- 刊名：Peptides
- 出版年：2012
- 期刊代码：59_01969781
- 类别：neu
- 出版时间：June, 2012
- 卷：35
- 期：2
- 页码：261-268
- 文件大小：1254 K
摘要
Peptide from croaker (Otolithes ruber) muscle protein hydrolysate was purified, characterized and evaluated for its in vitro and in vivo antioxidant activity. Results showed that purified peptide contained the amino acid sequence as Lys-Thr-Phe-Cys-Gly-Arg-His (861.6 Da), which were expected to contribute to its antioxidant activities. This peptide efficiently quenched 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals (84.5 卤 1.2 and 62.4 卤 2.9%), and successfully inhibits the lipid peroxidation and DNA damage and proven to be a potent antioxidant at different in vitro systems. It also improved the endogenous cellular antioxidant enzymes in Wistar rat by increasing the activities of catalase (CAT), glutathione-S-transferase (GST) and superoxide dismutase (SOD) after supplementation of the peptide (283.6 卤 7.25, 4.3 卤 0.78 and 28.42 卤 1.97) compared to the negative control (196.4 卤 5.65, 1.3 卤 0.45 and 15.1 卤 0.35). Therefore, croaker muscle peptide can increase an endurance capacity and facilitate recovery from oxidative stress.