Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family

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• 作者：Byeong-Gu Han^a ; ^d ; Kyung-Chae Jeong^a ; Jea-Won Cho^a ; Byung-Cheon Jeong^b ; Hyun Kyu Song^b ; Jae Young Lee^c ; Dong Hyuk Shin^d ; Sangho Lee^d ; Byung Il Lee^a ; ^{bilee@ncc.re.kr}
• 关键词：PF2050 ; DUF2666 ; DNA binding ; Pyrococcus furiosus
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：7 May, 2012
• 卷：586
• 期：9
• 页码：1384-1388
• 文件大小：1093 K

摘要

Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel 尾-sheet and an 伪-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.