Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchus Labrax L.)
- 作者：Francesco Buonocore ; Elisa R ; elli ; Steve Bird ; Chris J. Secombes ; Angelo Facchiano ; Susan Costantini ; Giuseppe Scapigliati
- 关键词：Interleukin-10 (IL-10) ; European sea bass ; Dicentrarchus labrax ; Expression analysis ; Real-time PCR ; 3D modelling
- 刊名：Aquaculture
- 出版年：2007
- 期刊代码：16_00448486
- 类别：abs
- 出版时间：28 September 2007
- 卷：270
- 期：1-4
- 页码：512-522
- 文件大小：802 K
摘要
In this paper we investigate the interleukin-10 (IL-10) expression and 3D modelling of the European sea bass (Dicentrarchus labrax L.). IL-10 is a regulatory cytokine that has been intensively studied in mammals and has been found mainly involved in the suppression or deactivation of activated immune responses. The full-length sea bass IL-10 cDNA consists of 1015 bp and is translated in a predicted molecule of 187 amino acids. A multiple alignment of this peptide with other known sequences showed the conservation of fundamental IL-10 family characteristics. Expression analysis by real-time PCR in control fish showed a high basal expression in the head kidney (HK), followed by gut and brain. In vitro treatment of HK leucocytes with LPS showed an intense increase of IL-10 expression at 4 h and a significant decrease at 24 h, with PHA-L no differences were evidenced. A 3D model for sea bass IL-10 was obtained by accurate homology procedures and putative interaction residues with the IL-10 receptor described. The results suggested that sea bass IL-10 could be involved in anti-inflammatory responses, but further experiments are needed to assess its importance in response to pathogenic agents, vaccinations and immunostimulants.