A new and selective and sensitive nanogold-labeled immunoresoance scattering spectral assay for trace prealbumin

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• 作者：Ming Hou ; Shuangjiao Sun ; Zhiliang Jiang
• 关键词：Gold nanoparticle labeling ; Immunological resonance scattering spectral probe ; Prealbumin
• 刊名：Talanta
• 出版年：2007
• 期刊代码：106_00399140
• 类别：ch
• 出版时间：30 April 2007
• 卷：72
• 期：2
• 页码：463-467
• 文件大小：331 K

摘要

A gold-labeled immunoresonance scattering spectral probe for trace prealbumin (PA) was prepared by using gold nanoparticles in size of 9.0 nm to label goat anti-human prealbumin polyclonal antibody. The immune reaction between the gold-labeled antibody and prealbumin took place in pH 7.6 Na₂HPO₄–NaH₂PO₄ buffer solution. In the presence of polyethylene glycol PEG-10000, the labeled gold nanoparticles were released and aggregated which brought the resonance scattering intensity (I_RS) at 580 nm to enhance greatly. The ΔI_RS is proportional to the prealbumin concentration in the range from 16.67 to 666.67 ng/mL, with a detection limit of 4.1 ng/mL. This simple, sensitive and selective assay was applied to determination of prealbumin in human plasma, with satisfactory results.