The influence of phase transitions in phosphatidylethanolamine models on the activity of violaxanthin de-epoxidase
- 作者：Astrid Vieler ; Holger A. Scheidt ; Peter Schmidt ; Cindy Montag ; Janine F. Nowoisky ; Martin Lohr ; Christian Wilhelm ; Daniel Huster ; Reimund Goss
- 关键词：Phospholipid structures ; 31P NMR ; Inverted hexagonal phase ; Arrhenius ; De-epoxidation ; Monogalactosyldiacylglycerol
- 刊名：Biochimica et Biophysica Acta (BBA)/Biomembranes
- 出版年：2008
- 期刊代码：14_00052736
- 类别：bio
- 出版时间：April 2008
- 卷：1778
- 期：4
- 页码：1027-1034
- 文件大小：262 K
摘要
In the present study, the influence of the phospholipid phase state on the activity of the xanthophyll cycle enzyme violaxanthin de-epoxidase (VDE) was analyzed using different phosphatidylethanolamine species as model lipids. By using 31P NMR spectroscopy, differential scanning calorimetry and temperature dependent enzyme assays, VDE activity could directly be related to the lipid structures the protein is associated with. Our results show that the gel (Lβ) to liquid-crystalline (L
) phase transition in these single lipid component systems strongly enhances both the solubilization of the xanthophyll cycle pigment violaxanthin in the membrane and the activity of the VDE. This phase transition has a significantly stronger impact on VDE activity than the transition from the L to the inverted hexagonal (HII) phase. Especially at higher temperatures we found increased VDE reaction rates in the presence of the L phase compared to those in the presence of HII phase forming lipids. Our data furthermore imply that the HII phase is better suited to maintain high VDE activities at lower temperatures.
) phase transition in these single lipid component systems strongly enhances both the solubilization of the xanthophyll cycle pigment violaxanthin in the membrane and the activity of the VDE. This phase transition has a significantly stronger impact on VDE activity than the transition from the L to the inverted hexagonal (HII) phase. Especially at higher temperatures we found increased VDE reaction rates in the presence of the L phase compared to those in the presence of HII phase forming lipids. Our data furthermore imply that the HII phase is better suited to maintain high VDE activities at lower temperatures.