Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2
- 作者：Bent O. Petersen ; Margit Sá ; ra ; Christoph Mader ; Harald F. Mayer ; Uwe B. Sleytr ; Martin Pabst ; Michael Puchberger ; Eberhard Krause ; AndreasHofinger ; Jens Ø ; . Duus ; Paul Kosma
- 关键词：Geobacillus ; Secondary cell wall polymer ; NMR spectroscopy ; Mass spectrometry ; Polysaccharide structure
- 刊名：Carbohydrate Research
- 出版年：2008
- 期刊代码：15_00086215
- 类别：ch
- 出版时间：9 June 2008
- 卷：343
- 期：8
- 页码：1346-1358
- 文件大小：1074 K
摘要
The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-mannose, and 2-acetamido-2-deoxy-d-mannuronic acid. The primary structure of the acid-degraded polysaccharide—liberated by HF-treatment from the cell wall—was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-
-d-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-d-glucopyranosyl units:
-d-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-d-glucopyranosyl units: