Syntheses and applications of fluorescent and biotinylated epolactaene derivatives: Epolactaene and its derivative induce disulfide formation
- 作者：Kouji Kuramochi ; Shunsuke Yukizawa ; Seiki Ikeda ; Takashi Sunoki ; Satoshi Arai ; Rie Matsui ; Akinori Morita ; Yoshiyuki Mizushina ; Kengo Sakaguchi ; Fumio Sugawara ; Masahiko Ikekita ; Susumu Kobayashi
- 关键词：Epoxides ; Proteins ; Antibiotics ; Binding proteins ; Reaction mechanism
- 刊名：Bioorganic and Medicinal Chemistry
- 出版年：2008
- 期刊代码：9_09680896
- 类别：ch
- 出版时间：1 May 2008
- 卷：16
- 期：9
- 页码：5039-5049
- 文件大小：400 K
摘要
Epolactaene, isolated from cultured Penicillium sp. BM 1689-P mycelium, induces neurite outgrowth and arrests the cell cycle of the human neuroblastoma cell line, SH-SY5Y, at the G1 phase. We have found that epolactaene and its derivatives induce apoptosis in the human leukemia B-cell line, BALL-1. In this study, we prepared fluorescent and biotinylated epolactaene derivatives. We characterized the cellular location and the identification of BALL-1 proteins that reacted with these compounds. The results obtained from the reaction of epolactaene or its derivative with N-acetylcysteine methyl ester indicate that these compounds induce the disulfide formation and the 
-position of the epoxylactam core is the reactive site.
-position of the epoxylactam core is the reactive site.