Novel amperometric glucose biosensor based on covalent immobilization of glucose oxidase on poly(pyrrole propylic acid)/Au nanocomposite

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• 作者：Mehmet 艦enel ; ^{msenel@fatih.edu.tr} ; Cevdet Nergiz ; ^{cnergiz@fatih.edu.tr}
• 关键词：Biosensor ; Glucose oxidase ; Pyrrole ; Immobilization ; Nanocomposite
• 刊名：Current Applied Physics
• 出版年：2012
• 期刊代码：15_15671739
• 类别：ph
• 出版时间：July, 2012
• 卷：12
• 期：4
• 页码：1118-1124
• 文件大小：978 K

摘要

A glucose biosensor is fabricated with immobilization of glucose oxidase (GOx) on poly(pyrrole propylic acid)/Au nanocomposite by covalent attachment. Poly(pyrrole propylic acid) (PPyAA)/Au nanocomposite was prepared by chemical oxidation of pyrrole propylic acid monomer by using chloroauric acid (HAuCl₄) as an oxidizing agent. The obtained nanocomposites were used to fabricate highly sensitive amperometric glucose biosensor which exhibited a high and reproducible sensitivity of 0.42 渭A/mM, response time 鈭? s, linear dynamic range from 1 to 18 mM, correlation coefficient of R² = 0.9981, and limit of detection (LOD), based on S/N ratio (S/N = 3) of 0.05 mM. A value of 1.83 mM for the apparent Michaelis-Menten constant was obtained. The high sensitivity, wider linear range, good reproducibility and stability make this biosensor a promising candidate for portable amperometric glucose biosensor.