Immobilization of enzyme on long period grating fibers for sensitive glucose detection
- 作者：Akash Deep ; Umesh Tiwari ; uktiwaricsio@yahoo.in ; Parveen Kumar ; Vandana Mishra ; Subhash C. Jain ; Nahar Singh ; Pawan Kapur ; Lalit M. Bharadwaj
- 关键词：Long period grating ; Glucose oxidase ; FTIR spectroscopy ; Raman spectroscopy ; Biosensing ; Glucose
- 刊名：Biosensors and Bioelectronics
- 出版年：2012
- 期刊代码：12_09565663
- 类别：ch
- 出版时间：15 March, 2012
- 卷：33
- 期：1
- 页码：190-195
- 文件大小：850 K
摘要
Glucose oxidase (GOD) immobilized long period grating (LPG) fibers have been proposed for the specific and sensitive detection of glucose. The treatment of LPG fibers with aminopropyl triethoxysilane has induced biding sites for the subsequent GOD immobilization. Field emission scanning electron microscopy, confocal laser scanning microscopy, infrared spectroscopy and Raman spectroscopy have provided detailed evidences about the effectiveness of the adopted biofunctionalization methodology. The enzyme activity is conserved during the immobilization step. Fabricated LPG sensor was tested on different glucose solutions to record the transmission spectra on an optical spectrum analyzer. The wavelength shifts in the transmission spectra are linearly correlated with the glucose concentration in the range of 10-300 mg dL鈭?. The fabricated sensor gives fast response and is demonstrated to be of practical utility by determining glucose contents in blood samples. Proposed technique can further be extended to develop LPG fiber based novel, sensitive and label free nanosensors for disease diagnosis and clinical analysis.