Role of PI3K伪 and sarcolemmal ATP-sensitive potassium channels in epoxyeicosatrienoic acid mediated cardioprotection

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• 作者：Sri N. Batchu^a ; Ketul R. Chaudhary^a ; Haitham El-Sikhry^a ; Wei Yang^b ; Peter E. Light^b ; Gavin Y. Oudit^c ; John M. Seubert^a ; ^b ; ^{jseubert@pharmacy.ualberta.ca}
• 关键词：EET ; pmKATP channel ; PI3K and Ischemia-Reperfusion
• 刊名：Journal of Molecular and Cellular Cardiology
• 出版年：2012
• 期刊代码：171_00222828
• 类别：bio
• 出版时间：July, 2012
• 卷：53
• 期：1
• 页码：43-52
• 文件大小：1077 K

摘要

Aims

Epoxyeicosatrienoic acids (EETs) are cytochrome P450 epoxygenase metabolites of arachidonic acid that have known cardioprotective properties. While the mechanism(s) remains unknown, evidence suggests that phosphoinositide 3-kinase (PI3K) and sarcolemmal ATP-sensitive potassium channels (pmK_ATP) are important. However the role of specific PI3K isoforms and corresponding intracellular mechanisms remains unknown.

Methods and results

To study this, mice hearts were perfused in Langendorff mode for 40 min of baseline and subjected to 20 or 30 min of global no-flow ischemia followed by 40 min of reperfusion. C57BL6 mice perfused with 11,12-EET (1 渭M) had improved postischemic recovery, whereas co-perfusion with PI3K伪 inhibitor, PI-103 (0.1 渭M), abolished the EET-mediated effect. In contrast, blocking of PI3K尾 or PI3K纬 isoforms failed to inhibit EET-mediated cardioprotection. In addition to the improved post-ischemic recovery, increased levels of p-Akt, decreased calcineurin activity and decreased translocation of proapoptotic protein BAD to mitochondria were noted in EET-treated hearts. Perfusion of 11,12-EET to Kir6.2 deficient mice (pmK_ATP) failed to improve postischemic recovery, decrease calcineurin activity and translocation of proapoptotic protein BAD, however increased levels of p-Akt were still observed. Patch-clamp experiments demonstrated that 11,12-EET could not activate pmK_ATP currents in myocytes pre-treated with PI-103. Mechanistic studies in H9c2 cells demonstrate that 11,12-EET limits anoxia-reoxygenation triggered Ca^2 + accumulation and maintains mitochondrial 螖唯m compared to controls. Both PI-103 and glibenclamide (10 渭M, pmK_ATP inhibitor) abolished EET cytoprotection.

Conclusion

Together our data suggest that EET-mediated cardioprotection involves activation of PI3K伪, upstream of pmK_ATP, which prevents Ca^2 + overload and maintains mitochondrial function.