Nanobiocomposite platform based on polyaniline-iron oxide-carbon nanotubes for bacterial detection

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• 作者：Renu Singh^a ; ^c  ; [Author Vitae] ; Rachna Verma^b  ; [Author Vitae] ; G. Sumana^a  ; [Author Vitae] ; Avanish Kumar Srivastava^a  ; [Author Vitae] ; Seema Sood^b  ; [Author Vitae] ; Rajinder K. Gupta^c  ; [Author Vitae] ; B.D. Malhotra^a ; ^d ; ^{bansi.malhotra@gmail.com}  ; [Author Vitae]
• 关键词：Electrochemical genosensor ; Neisseria gonorrhoeae ; Sexually transmitted disease ; Nanocomposite ; Nucleic acid hybridization
• 刊名：Bioelectrochemistry
• 出版年：2012
• 期刊代码：7_15675394
• 类别：ch
• 出版时间：August, 2012
• 卷：86
• 期：Complete
• 页码：30-37
• 文件大小：2191 K

摘要

The nanocomposite based on polyaniline (PANI)-iron oxide nanoparticles (nFe₃O₄) and multi walled carbon-nanotubes (CNT) has been fabricated onto indium tin oxide (ITO) coated glass plate via facile electrochemical synthesis of polyaniline in presence of nFe₃O₄ (~ 20 nm) and CNT (20-80 nm in diameter). The results of transmission electron microscopic studies show evidence of coating of PANI and nFe₃O₄ onto the CNT. The PANI-nFe₃O₄-CNT/ITO nanoelectrode has been characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy studies. The biotinylated nucleic acid probe sequence consisting of 20 bases has been immobilized onto PANI-nFe₃O₄-CNT/ITO nanoelectrode using biotin-avidin coupling. It is shown that the PANI-nFe₃O₄-CNT platform based biosensor can be used to specifically detect bacteria (N. gonorrhoeae) at minute concentration as low as (1 脳 10^鈭?#xA0;19 M) indicating high sensitivity within 45 s of hybridization time at 298 K by differential pulse voltammetry using methylene blue as electroactive indicator. This bacterial sensor has also been tested with 4 positive and 4 negative PCR amplicons of gonorrhoea affected patient samples. The results of these studies have implications towards the fabrication of a handheld device for Neisseria gonorrhoeae detection that may perhaps result in a decrease in the human immunodeficiency virus infections.