A separation-free amperometric immunosensor for vitellogenin based on screen-printed carbon arrays modified with a conductive polymer
- 作者：Darain ; Farzana ; Park ; Doeg Su ; Park ; Jang-Su ; Chang ; Seung-Cheol ; Shim ; Yoon-Bo
- 关键词：Screen-printed carbon arrays ; Vitellogenin ; Conducting polymer ; Amperometric immunosensor ; Mediatorless system ; Enzyme label
- 刊名：Biosensors and Bioelectronics
- 出版年：2005
- 期刊代码：12_09565663
- 类别：ch
- 出版时间：March 15, 2005
- 卷：20
- 期：9
- 页码：1780-1787
- 文件大小：328 K
摘要
A disposable amperometric immunosensor was studied for the rapid detection of carp (Carassius auratus) Vitellogenin (Vtg). The sensor was fabricated based on screen-printed carbon arrays (SPCAs) containing eight carbon working and an integrated carbon counter electrodes. To construct the sensor, a conducting polymer (poly-terthiophene carboxylic acid) was electropolymerized on the surface of working electrodes and the polymer-coated SPCAs was characterized by SEM. Horseradish peroxidase (HRP) and a monoclonal antibody (anti-Vtg) specific to carp Vtg were covalently attached onto the polymer modified SPCAs. The immobilization of HRP and anti-Vtg onto the polymer-coated SPCAs was examined using cyclic voltammetry and quartz crystal microbalance studies. In order to detect the amount of Vtg, glucose oxidase (GOx)-labelled Vtg bound to the sensor surface under competition with the Vtg analyte was quantified amperometrically using glucose as a substrate. The performance of the eight sensors in arrays was evaluated by obtaining the calibration plots for Vtg. The sensor arrays exhibit a linear range of the Vtg concentration from 0.25 to 7.8ng/ml and the detection limit was determined to be 0.09ng/ml. Furthermore, the performance of the immunosensor for the determination of Vtg was evaluated by a standard addition method performed in fish serum samples.