Evaluation of 9-[(3-¹⁸F-fluoro-1-hydroxy-2-propoxy)methyl]guanine ([¹⁸F]-FHPG) in vitro and in vivo as a probe for PET imaging of gene incorporation and expression

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• 作者：Alauddin ; Mian M. ; Shahinian ; Antranik ; Kundu ; Ramendra K. ; Gordon ; Erlinda M. ; Conti ; Peter S.
• 关键词：[¹⁸F]-FHPG ; PET imaging ; Gene incorporation
• 刊名：Nuclear Medicine and Biology
• 出版年：1999
• 期刊代码：43_09698051
• 类别：ph
• 出版时间：May, 1999
• 卷：26
• 期：4
• 页码：371-376
• 文件大小：361 K

摘要

Preparation of 9-[(3-¹⁸F-fluoro-1-hydroxy-2-propoxy)methyl]-guanine ([¹⁸F]-FHPG) for clinical use, and its evaluation as a positron emission tomography (PET) imaging agent for gene incorporation and expression in tumors are reported. In vitro studies in human colon cancer cells, HT-29, transduced with the retroviral vector G1Tk1SvNa and nontransduced (wild type) showed 4, 8, 12, and 15 times higher uptake of the probe in 1, 3, 5, and 7 h, respectively, in transduced cells compared with the controls. In vivo studies in tumor-bearing nude mice demonstrated that the tumor uptake of the radiotracer is three and six-fold higher in 2 and 5 h, respectively, in transduced cells compared with the control cells. These results suggest that [¹⁸F]-FHPG is a potential in vivo PET imaging agent for monitoring gene incorporation and expression in gene therapy of cancer.