Purification of a polysaccharide from Gynostemma pentaphyllum Makino and its therapeutic advantages for psoriasis

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• 作者：Xiao-Li Li^a ; ^{xiaolili.xjtu.edu.cn@gmail.com} ; Zheng-Hui Wang^b ; ; Yong-Xi Zhao^c ; Su-Ju Luo^d ; Ding-Wei Zhang^a ; Sheng-Xiang Xiao^a ; Zhen-Hui Peng^a
• 关键词：Gynostemma pentaphyllum ; Polysaccharide ; Purification ; Psoriasis
• 刊名：Carbohydrate Polymers
• 出版年：2012
• 期刊代码：14_01448617
• 类别：ch
• 出版时间：1 August, 2012
• 卷：89
• 期：4
• 页码：1232-1237
• 文件大小：570 K

摘要

In current study, a water-soluble polysaccharide (GP-I), with a molecular mass of 33 kDa, was purified from Gynostemma pentaphyllum. Gas chromatography (GC) analysis suggested that it was composed of Glc, Gal, Man, Rha and Ara with a ratio of 5.3: 4.2: 3.0: 0.7: 0.8. The GP-I (25, 50, 100, 200 and 400 渭g/ml) was found to have significant anti-proliferative effects on HaCat cells in a dose-dependent manner, as measured by MTT assay. On the contrary, Trypan blue exclusion experiment indicated that GP-I had no cytotoxicity to HaCat cells. Moreover, the decrease of mitochondrial membrane potential (MMP) in GP-I treated cells was also observed, indicating apoptosis in HaCat cells. Besides, tumor necrosis factor-伪 (TNF-伪), a vital pro-inflammatory cytokine in psoriasis, in the supernatant of HaCat cells was dramatically reduced by GP-I. Collectively, these findings suggested that GP-I was a promising agent to be developed for psoriasis treatment in clinical therapy.