Isolation and antitumor activities of acidic polysaccharide from Gynostemma pentaphyllum Makino

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• 作者：Xiao-Li Li^a ; ^{xiaolili.xjtu.edu.cn@gmail.com} ; Zheng-Hui Wang^b ; ; Yong-Xi Zhao^c ; Su-Ju Luo^d ; Ding-Wei Zhang^a ; Sheng-Xiang Xiao^a ; Zhen-Hui Peng^a
• 关键词：Gynostemma pentaphyllum ; Acidic polysaccharide ; Purification ; Antitumor ; Melanoma
• 刊名：Carbohydrate Polymers
• 出版年：2012
• 期刊代码：14_01448617
• 类别：ch
• 出版时间：1 July, 2012
• 卷：89
• 期：3
• 页码：942-947
• 文件大小：376 K

摘要

Two acidic polysaccharides (GP-B1 and GP-C1) were obtained from Gynostemma pentaphyllum. The molecular weights (Mw) of the two fractions were 79 kDa for GP-B1 and 126 kDa for GP-C1. GP-B1 was composed of Gal, Ara, Man, Rha, Xyl, Glc, GalA and GlcA in a molar ration of 3.5:3.2:0.6:0.9:0.3:0.5:0.6:0.4. GP-C1 consisted of Gal, Ara, Man, Rha, Glc, and GlcA in the proportions of 2.1:1.0:0.3:0.5:0.4:0.9. Among them, GP-B1 treatment had a significant inhibitory effect on the growth of melanoma B16 in vivo and in vitro. Meanwhile GP-B1 could increase the relative spleen weight and stimulate the splenocyte proliferation alone or combined with ConA. Moreover, GP-B1 treatment induced an evident increase in the level of serum TNF-伪, IFN-纬, and IL-12 and a reduction for IL-10 production. These results indicate that the antitumor effects of GP-B1 are associated with immunostimulation.