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The effects of different KI intake on H2O2 content, calcium channel and anti-oxidative ability in FRTL cells
In leaves, six isoforms of SOD were recognized. Roots possessed two additional bands, named manganese superoxide dismutase (MnSOD)-like form (MnSODI) and Cu/ZnSOD-like form (Cu/ZnSODIV). The H2O2 content in leaves ranged from 554 to 5 098 μmol H2O2/g f.w. and was negatively correlated with CAT activity. The non-contaminated population was characterized by the lowest CAT activity combined with the highest H2O2 concentration. Two isoforms of CAT, CAT-1 and CAT-2, were recognized in leaves of plants from non-contaminated and contaminated sites, respectively. In roots of individuals from two heaps (‘Warpie’ and ‘Saturn’), two distinct bands for each CAT isoform were observed. A slower migrating band may be an aggregate, exhibiting CAT and MnSODs activities. Both peroxidases (APX and GPX) presented the same pattern of activity, depending on the organ, indicating that in leaves and roots APX and GPX were regulated in parallel.
Differences in enzyme activities and H2O2 content between plants from different contaminated sites were statistically significant, but were tightly maintained at a very similar level. Prolonged and permanent heavy metal stress evoked a very similar mode of antioxidative response in specimens of analyzed metalliferous populations not causing measurable oxidative stress. Thus, our results clearly indicate that V. tricolor is a taxon well adapted to heavy metal-contaminated soils, and that differences in enzyme activities and H2O2 content result from adjustment of plants to a variety of conditions.
New macroporous calcium phosphate glass ceramic for gui... Biomaterials |
New macroporous calcium phosphate glass ceramic for guided bone regeneration Biomaterials, Volume 25, Issue 18, August 2004, Pages 4233-4241 Melba Navarro, Sergio del Valle, Salvador Martínez, Stefania Zeppetelli, Luigi Ambrosio, Josep Anton Planell, Maria Pau Ginebra Abstract This work describes a method to obtain macroporous resorbable glass and glass ceramic scaffolds with controlled biodegradability for tissue engineering applications. The constructs consisted of glass and glass ceramics in the system P2O5–CaO–Na2O–TiO2 and they were prepared by foaming a slurry of glass particles by addition of a H2O2 solution, and subsequent sintering of the porous structures obtained. Different thermal treatments were applied to control the degree of devitrification of the glass. The resultant materials showed a porosity percentage between 40%and 55%with a wide variety of pores ranging from 20 to 500 μm in diameter as determined by SEM and Image Analysis. The resulting constructs were predominantly formed by a vitreous phase, although small amounts of calcium metaphosphate and pyrophosphates were detected by X-ray diffraction and Raman spectroscopy after the sintering process. The biological response was also evaluated by means of the MTT test, the material showed a non-cytotoxic effect. Purchase PDF (558 K) |
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The effects of different KI intake on H2O2 content, calcium channel and anti-oxidative ability in FRTL cells