Identification and characterization of zebrafish semaphorin 6D
- 作者：Masafumi Kimura ; Masahiko Taniguchi ; Yoshinori Mikami ; Tomoyuki Masuda ; Tomoyuki Yoshida ; Masayoshi Mishina ; Takao Shimizu
- 关键词：Semaphorin ; Plexin ; Axon guidance ; Brain ; Lens ; Rhombomere ; Repellent
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：23 November 2007
- 卷：363
- 期：3
- 页码：762-768
- 文件大小：1294 K
摘要
The semaphorin gene family contains a large number of secreted and transmembrane proteins; some function as repulsive and attractive cues of axon guidance during development. Here, we report cloning and characterization of zebrafish transmembrane semaphorin gene, semaphorin 6D (sema6D). Sema6D is expressed predominantly in the nervous system during embryogenesis, as determined by in situ hybridization. We also found that Sema6D binds Plexin-A1 in vitro, but not other Plexins. It induces the repulsion of dorsal root ganglion axons, but not sympathetic axons. Consequently, Sema6D might use Plexin-A1 as a receptor to repel specific types of axons during development.