Labdane-type diterpenoids from hairy root cultures of Coleus forskohlii, possible intermediates in the biosynthesis of forskolin

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• 作者：Yoshihisa Asada^a ; ^{asaday@rs.noda.tus.ac.jp} ; Wei Li^b ; ^{liwei@phar.toho-u.ac.jp} ; Tomohiro Terada^c ; Xinzhu Kuang^b ; Qin Li^d ; Takafumi Yoshikawa^c ; Shogo Hamaguchi^b ; Iyuki Namekata^b ; Hikaru Tanaka^b ; Kazuo Koike^b
• 关键词：Hairy root ; Coleus forskohlii ; Labiatae ; Diterpene ; Forskolin
• 刊名：Phytochemistry
• 出版年：2012
• 期刊代码：85_00319422
• 类别：ch
• 出版时间：July, 2012
• 卷：79
• 期：Complete
• 页码：141-146
• 文件大小：632 K

摘要

Significant attention has been devoted to studying hairy root cultures as a promising strategy for production of various valuable secondary metabolites. These offer many advantages, such as high growth rate, genetic stability and being hormone-free. In this study, a detailed phytochemical investigation of the secondary metabolites of Coleus forskohlii hairy root cultures was undertaken and which resulted in the isolation of 22 compounds, including four forskolin derivatives and a monoterpene. Their structures were elucidated by extensive spectroscopic analyses. These compounds could be classified into four groups viz.: labdane-type diterpenes, monoterpenes, triterpenes and phenylpropanoid dimers. Apart from one compound, all labdane type diterpenes are oxygenated at C-11 as in forskolin and a scheme showing their biosynthetic relationships is proposed.