Rapid aggregation of heterogeneous cells and multiple-sized microspheres in methylcellulose medium

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• 作者：Nobuhiko Kojima^a ; ^b ; ^{nobuhiko@iis.u-tokyo.ac.jp} ; Shoji Takeuchi^a ; ^b ; Yasuyuki Sakai^a ; ^b
• 关键词：Swelling ; Absorption ; Microsphere ; Nanoparticle
• 刊名：Biomaterials
• 出版年：2012
• 期刊代码：7_01429612
• 类别：ms
• 出版时间：June, 2012
• 卷：33
• 期：18
• 页码：4508-4514
• 文件大小：1333 K

摘要

We report a method for the rapid production of cellular aggregates without electric power and cell modification. We focused on the swelling property of a solution containing a high molecular material, methylcellulose (MC), which immediately absorbs a small amount of solvent and fills the space occupied by the solvent. When 1聽渭l of a suspension of 1000 animal cells in normal culture medium was injected into the 3%MC medium, the normal medium was rapidly absorbed by the surrounding MC medium. Suspended cells were simultaneously trapped on the interfaces between the normal and MC media; they were finally pulled together and held in the MC medium. This event was nearly complete within the first 10聽min. Moreover, MC medium-dependent aggregation was observed when polystyrene microspheres of different sizes (diameter, 100聽nm-100聽渭m) were added. Furthermore, we demonstrated the stepwise fabrication of multi-layered aggregates with embedded structures. These methods for creating engineered aggregates should enhance the study of three-dimensional cultures comprising two or more cell types with well-designed structures.