Shp1, a regulator of protein phosphatase 1 Glc7, has important roles in cell morphogenesis, cell cycle progression and DNA damage response in Candida albicans

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• 作者：Kangdi Hu^a ; Wanjie Li^a ; Haitao Wang^a ; Kun Chen^a ; Yue Wang^b ; ^{mcbwangy@imcb.a-star.edu.sg} ; Jianli Sang^a ; ^{jlsang@bnu.edu.cn}
• 关键词：SHP1 ; Glc7/protein phosphatase 1 ; Cell morphogenesis ; Cell cycle ; DNA damage response
• 刊名：Fungal Genetics and Biology
• 出版年：2012
• 期刊代码：116_10871845
• 类别：abs
• 出版时间：June, 2012
• 卷：49
• 期：6
• 页码：433-442
• 文件大小：1507 K

摘要

In yeast, the type 1 protein phosphatase (PP1) catalytic subunit Glc7 is involved in the regulation of multiple cellular processes and thought to achieve specificity through association with different regulatory subunits. Here, we report that the Glc7 regulator Shp1 plays important roles in cell morphogenesis, cell cycle progression and DNA damage response in Candida albicans. SHP1 deletion caused the formation of rod-shaped yeast cells with slow growth. Flow cytometry analysis revealed that shp1螖 cells showed a prolonged G₂/M phase, which was rescued by deleting the spindle-checkpoint gene MAD2. Furthermore, shp1螖 cells were hypersensitive to heat and genotoxic stresses. Interestingly, depletion of Glc7 caused defects similar to the shp1螖 mutant such as arrest at G₂/M transition; and the GLC7/glc7螖 heterozygous mutant exhibited increased sensitivity to genotoxic stresses, consistent with the recent finding that Saccharomyces cerevisiae Glc7 has a role in DNA damage response. We also show that Shp1 is required for the nuclear accumulation of Glc7, suggesting that Shp1 executes its cellular function partly by regulating Glc7 localization.