Simultaneous determination of 10 major flavonoids in Dalbergia odorifera by high performance liquid chromatography

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• 作者：Rong-Xia Liu ; Qiao Wang ; Hong-Zhu Guo ; Li Li ; Kai-Shun Bi and De-An Guo
• 关键词：Dalbergia odorifera ; Flavonoids ; Reversed-phase high performance liquid chromatography
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2005
• 期刊代码：65_07317085
• 类别：ch
• 出版时间：2005
• 卷：39
• 期：3-4
• 页码：469-476
• 文件大小：319 K

摘要

A reversed-phase liquid chromatographic method was developed for the simultaneous quantification of 10 major flavonoids, namely butin, (3R)-4′-methoxy-2′,3,7-trihydroxyisoflavanone, liquiritigenin, melanettin, violanone, vistitone, formononetin, dalbergin, sativanone and medicarpin in the heartwood of Dalbergia odorifera, an important traditional Chinese medicine. Samples were extracted with 60%methanol. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C₁₈ column (250 mm × 4.6 mm, 5 μm) with a gradient of acetonitrile and 0.3%(v/v) aqueous acetic acid, at a flow rate of 0.8 ml/min, detected at 275 nm. The complete separation was obtained within 55 min for the 10 target compounds. All calibration curves showed good linearity (r² > 0.999) within test ranges. The assay was reproducible with overall intra- and inter-day variation of less than 3%. The mean recovery of the method was 100 ± 10%, with R.S.D. less than 5%. The current assay method was considered to be suitable for the quality control of D. odorifera samples and could be readily utilized for the determination of the active principles present in this medicinal herb.