Reverse genetic studies of the DNA damage response in the chicken B lymphocyte line DT40

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• 作者：Yamazoe ; Mitsuyoshi ; Sonoda ; Eiichiro ; Hochegger ; Helfrid ; Takeda ; Shunichi
• 关键词：DT40 ; Gene disruption ; Conditional mutant ; Homologous DNA recombination ; Genome instability
• 刊名：DNA Repair
• 出版年：2004
• 期刊代码：121_15687864
• 类别：ch
• 出版时间：August - September, 2004
• 卷：3
• 期：8-9
• 页码：1175-1185
• 文件大小：289 K

摘要

In the ‘post-genome’ era, reverse genetics is one of the most informative and powerful means to investigate protein function. The chicken B lymphocyte line DT40 is widely used for reverse genetics because the cells have a number of advantages, including efficient gene targeting as well as a remarkably stable phenotype. Furthermore, the absence of functional p53 in DT40 cells enables identification of DNA damage using chromosome analysis by suppressing damage-induced apoptosis during interphase. This review summarizes the contribution of DT40 cells to reverse genetic studies of DNA damage response pathways in higher eukaryotic cells.