Protective effect of yacon leaves decoction against early nephropathy in experimental diabetic rats

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• 作者：Stella M. Honoré ; ¹ ; Wilfredo M. Cabrera¹ ; Susana B. Genta ; Sara S. Sá ; nchez ; ^{ssanchez@fbqf.unt.edu.ar}
• 关键词：Diabetes ; Kidney ; Yacon ; TGF/Smad signaling
• 刊名：Food and Chemical Toxicology
• 出版年：2012
• 期刊代码：20_02786915
• 类别：pha
• 出版时间：May, 2012
• 卷：50
• 期：5
• 页码：1704-1715
• 文件大小：3372 K

摘要

Nephropathy is the most common cause of morbidity and mortality in diabetic patients. Prevention of this complication has a major relevance. Smallanthus sonchifolius (yacon) leaves have been shown to ameliorate hyperglycemia in streptozotocin-induced diabetic rats. We examined the beneficial effects of yacon leaves decoction on diabetic nephropathy and explored the possible underlying action mechanism.

Streptozotocin-diabetic rats were orally administered 10%yacon leaves water decoction (70 mg dry extract/kg body weight) once a day for 4 weeks. Biochemical parameters in blood and urine were analyzed and immunohistochemistry staining, western immunoblotting and qRT-PCR were assessed.

Yacon decoction significantly decreased high blood glucose level in diabetic rats and improved insulin production. Diabetic-dependent alterations in urinary albumin excretion, creatinine clearance, kidney hypertrophy and basement membrane thickening were attenuated by yacon decoction. These findings were associated with a marked decrease in TGF-尾1/Smad2/3 signaling. The expression of molecular markers of diabetic nephropathy such as collagen IV, laminin-1, fibronectin and collagen III were also diminished in the yacon-treated group compared to control diabetic group. These results suggest that yacon leaves decoction is a protective agent against renal damage in diabetic nephropathy, whose action can be mediated by TGF-尾/Smads signals.