Use of chitosan-based edible coatings in combination with other natural compounds, to control Rhizopus stolonifer and Escherichia coli DH5伪 in fresh tomatoes

详细信息	查看全文 | 推荐本文 |

• 作者：Margarita Ramos-Garc&iacute ; a^a ; Elsa Bosquez-Molina^b ; Jesú ; s Herná ; ndez-Romano^c ; Guadalupe Zavala-Padilla^d ; Eduardo Terré ; s-Rojas^e ; Irá ; n Alia-Tejacal^f ; Laura Barrera-Necha^a ; Mó ; nica Herná ; ndez-Ló ; pez^a ; Silvia Bautista-Bañ ; os^a ; ^{sbautis@ipn.mx}
• 关键词：Lime essential oil ; Beeswax ; Oleic acid ; Lycopersicon esculentum ; Food-borne pathogens
• 刊名：Crop Protection
• 出版年：2012
• 期刊代码：28_02612194
• 类别：abs
• 出版时间：August, 2012
• 卷：38
• 期：Complete
• 页码：1-6
• 文件大小：363 K

摘要

During storage of tomatoes, Rhizopus stolonifer rapidly spreads towards adjacent fruits causing severe economic losses while Escherichia coli may cause serious even life threatening diseases. Chitosan-based materials can be used as edible films or coatings to avoid water loss and microbial spoilage. Waxes and essential oils may also be considered for use as antimicrobial agents in chitosan coating. In this study, various chitosan-based formulations (1%) mixed with beeswax (0.1%), oleic acid (1.0%), and lime or thyme essential oil (0.1%) were tested on tomato at three different maturity stages to control R.聽stolonifer and E.聽coli DH5伪 at storage temperatures of 12聽掳C and 25聽掳C. Control fruit were only dipped in water. Overall, fruit were wounded, coated and inoculated. Once the coatings were applied, 20聽渭l of R.聽stolonifer spore suspension at 10⁵ spores ml^鈭? concentration and 35聽渭l of bacterial solution of E.聽coli DH5伪, at 10⁵聽cfu聽渭l^鈭?, concentration, were dispensed over the wounded surface. Experiments were carried out in聽vitro, at a small scale and at semi commercial level. Overall, the protection effect of coating applications was better against E.聽coli DH5伪 than R.聽stolonifer. For in聽vitro experiments the best coatings was that of chitosan (1%)聽+聽beeswax (0.1%)聽+聽lime essential oil (0.1%) since no growth of R.聽stolonifer and E.聽coli DH5伪 took place. Other coating that stopped R.聽stolonifer growth was that of chitosan (1%)聽+聽oleic acid (1%)聽+聽lime essential oil (0.1%) while for E.聽coli DH5伪 were chitosan (1%)聽+聽beeswax (0.1%)聽+聽thyme essential oil (1%) and chitosan (1%)聽+聽beeswax (0.1%). Observation with an electronic scanning microscope showed distorted mycelia and no development of R.聽stolonifer sporangiospores, and no growth of E.聽coli DH5伪 when both microorganisms were grown on the formulation of chitosan (1%)聽+聽beeswax (0.1%)聽+聽lime essential oil (0.1%). For E.聽coli DH5伪, this same formulation applied on tomatoes at a small scale and in the semi commercial level completely controlled E.聽coli DH5伪 at both storage temperatures. The application of chitosan-based edible coating containing beeswax and lime essential oil is promising to follow since it is an environmentally-friendly alternative to control this important pathogenic microorganism. Export tomato producers might benefit from this nonchemical alternative.