Two-stage bile preparation with acetone for recovery of fluorescent aromatic compounds (FACs)

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• 作者：Ali Karami^a ; ^{alikaramiv@gmail.com} ; Mohd A. Syed^b ; Annie Christianus^a ; Kristine L. Willett^c ; Jeffrey R. Mazzeo^d ; Simon C. Courtenay^e
• 关键词：PAHs ; polycyclic aromatic hydrocarbons ; HPLC/FL ; high-performance liquid chromatography with fluorescent detection (HPLC/FL) ; FACs ; fluorescent aromatic compounds ; L&ndash ; LE ; liquid&ndash ; liquid extraction ; 7 ; 8-D BaP ; 7 ; 8dihydrodiolbenzo[a]pyrene ; 1-OH B
• 刊名：Journal of Hazardous Materials
• 出版年：2012
• 期刊代码：50_03043894
• 类别：ce
• 出版时间：15 July, 2012
• 卷：223-224
• 期：Complete
• 页码：84-93
• 文件大小：429 K

摘要

In this study we sought to optimize recovery of fluorescent aromatic compounds (FACs) from the bile of African catfish (Clarias gariepinus) injected with 10 mg/kg benzo[a]pyrene (BaP). Fractions of pooled bile were hydrolyzed, combined with ten volumes of methanol, ethanol, acetonitrile, or acetone, centrifuged and supernatants were analyzed by high-performance liquid chromatography with fluorescent detection (HPLC/FL). As well, to test whether FACs were being lost in solids from the centrifugation, pellets were resuspended, hydrolyzed and mixed with six volumes of the organic solvent that produced best FAC recovery from the supernatant, and subjected to HPLC/FL. Highest FAC concentrations were obtained with 2000 渭l and 1250 渭l acetone for supernatants and resuspended pellets respectively. FACs concentrations were negatively correlated with biliary protein content but were unaffected by addition of bovine serum albumin (BSA) followed by no incubation indicating that the presence of proteins in the biliary mixture does not simply interfere with detection of FACs. In another experiment, efficiency of acetone addition was compared to two different liquid-liquid extractions (L-LEs). Acetone additions provided significantly higher biliary FACs than the L-LE methods. The new two-stage bile preparation with acetone is an efficient, inexpensive and easily performed method.