Interaction between toxigenic fungi and weevils in corn grain samples
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摘要
This study investigated the ability of weevils to transmit Aspergillus flavus and Fusarium verticillioides fungal spores and the consequent production of mycotoxins. For this purpose, corn grain samples were stored in flasks connected to a hose to form a closed system (flasks A and B). Flasks A were inoculated with the following groups: group 1 (corn聽+聽weevil); group 2 (corn聽+聽A.聽flavus); group 3 (corn聽+聽A.聽flavus聽+聽weevil); group 4 (corn聽+聽F.聽verticillioides); group 5 (corn聽+聽F.聽verticillioides聽+聽weevil), and group 6 (corn聽+聽A.聽flavus聽+聽F.聽verticillioides聽+聽weevil). Flasks B contained sterile grains. The samples were incubated for 10, 20 and 30 days, posteriorly, weight, water activity, mycoflora, aflatoxins and fumonisins. The corn grain samples were also submitted to scanning electron microscopy. Our results showed that weevils could enhance corn grains contamination by these fungi, hence, could increase mycotoxins production. These findings demonstrate the importance of weevils as fungal vectors and the need for good manipulation and storage practices of grains.

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