The effects of aging, physical training, and a single bout of exercise on mitochondrial protein expression in human skeletal muscle

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• 作者：Zoltan Bori^a ; Zhongfu Zhao^a ; ^b ; Erika Koltai^a ; Ioannis G. Fatouros^c ; Athanasios Z. Jamurtas^d ; Ioannis I. Douroudos^c ; Gerasimos Terzis^e ; Athanasios Chatzinikolaou^c ; Apostolos Sovatzidis^f ; Dimitrios Draganidis^c ; Istvan Boldogh^g ; Zsolt Radak^a ; ^radak@tf.hu
• 关键词：Aging ; Exercise ; Skeletal muscle ; Mitochondrial biogenesis ; Fusion and fission
• 刊名：Experimental Gerontology
• 出版年：2012
• 期刊代码：99_05315565
• 类别：med
• 出版时间：June, 2012
• 卷：47
• 期：6
• 页码：417-424
• 文件大小：712 K

摘要

Aging results in a significant decline in aerobic capacity and impaired mitochondrial function. We have tested the effects of moderate physical activity on aerobic capacity and a single bout of exercise on the expression profile of mitochondrial biogenesis, and fusion and fission related genes in skeletal muscle of human subjects. Physical activity attenuated the aging-associated decline in VO2 max (p < 0.05). Aging increased and a single exercise bout decreased the expression of nuclear respiratory factor-1 (NRF1), while the transcription factor A (TFAM) expression showed a strong relationship with VO_2max and increased significantly in the young physically active group. Mitochondrial fission representing FIS1 was induced by regular physical activity, while a bout of exercise decreased fusion-associated gene expression. The expression of polynucleotide phosphorylase (PNPase) changed inversely in young and old groups and decreased with aging. The A2 subunit of cyclic AMP-activated protein kinase (AMPK) was induced by a single bout of exercise in skeletal muscle samples of both young and old subjects (p < 0.05). Our data suggest that moderate levels of regular physical activity increases a larger number of mitochondrial biogenesis-related gene expressions in young individuals than in aged subjects. Mitochondrial fission is impaired by aging and could be one of the most sensitive markers of the age-associated decline in the adaptive response to physical activity.