Spiral coils for counter-current chromatography using aqueous polymer two-phase systems
- 作者：Y.H. Guan ; J. Smulders ; D. Fisher ; I.A. Sutherl
- 关键词：Counter-current chromatography ; Aqueous two-phase systems ; Spiral column holders ; Polyethylene glycol ; PEG ; Stroboscopic imaging
- 刊名：Journal of Chromatography A
- 出版年：2007
- 期刊代码：47_00219673
- 类别：ch
- 出版时间：1 June 2007
- 卷：1151
- 期：1-2
- 页码：115-120
- 文件大小：658 K
摘要
Retention properties of polyethylene glycol–phosphate aqueous two-phase systems in a spiral coil (5 mm I.D.) on Type-J synchronous counter-current chromatographic devices have been compared for the elution mode where the lower phase is the mobile phase and flows from the inside head terminal. This was achieved with the aid of digital imaging under stroboscopic illumination, an image analysis and measurement of the displaced volume of the stationary phase. For the spiral coil, high and stable stationary phase retention at mobile phase flow rates up to 64 ml/min has been obtained. Wave-like disturbance of the interface near the proximal point was observed and analyses have been made for possible use in protein separation.