Mechanism of vitamin D₃-induced transcription of phospholipase D1 in HaCat human keratinocytes

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• 作者：Ryosuke Kikuchi ; Sayaka Sobue ; Masashi Murakami ; Hiromi Ito ; Ami Kimura ; Takashi Iwasaki ; Shuji Shibayama ; Akira Takagi ; Tetsuhito Kojima ; Motoshi Suzuki ; et al.
• 关键词：1α ; 25-Dihydroxyvitamin D₃ ; PLD1 gene expression ; Promoter analysis ; VDR ; RXRα
• 刊名：FEBS Letters
• 出版年：2007
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：1 May 2007
• 卷：581
• 期：9
• 页码：1800-1804
• 文件大小：173 K

摘要

1α,25-Dihydroxyvitamin D₃ (VitD₃) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD₃ increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (−260 bp to −246 bp from exon 1). Similar results were obtained by transfecting VitD₃ receptor (VDR) into HEK293 cells, which are originally VitD₃-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD₃, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.